ENZIM Biotech | Biophosphorin phosphorus-kaliy mobilizer

description

Biophosphorin - biotechnological preparation of prolonged action to improve the phosphorus and potassium nutrition of plants and stimulate the growth of the root system.

Active substance:: Living cells and bacterial spores Bacillus megaterium strain BM 206 with a title not less 5х10⁸ CFU/ml (dry form of spore not less than 1х10⁹ КУЕ/г) and products of their metabolism (phytohormones of the auxin, giberellin and cytokinin series, amino acids, vitamins).
Preparative form:: Liquid / Dry form.
Storage conditions:: Store at t° from + 2 °С to + 15 °С in a dark place protected from direct sunlight.
Shelf life:: 8 months (dry form 12 months).

mechanism

The mechanism of action of the preparation Biophosphorin on plants

splitting of poorly soluble soil phosphorus compounds

the strain has significant phosphatase activity - 190 - 340 μM phenolphthalein phosphate per 1 g of biomass per hour

сsynthesis of various biological active substances such as phytohormones of the auxin series

the producer of the preparation is able to synthesize an average of 1.5 - 2.36 μg / ml of indoleacetic acid, which contributes to the better development of the root system of agricultural crops

the ability to break down chemical pesticide residues

when cultivating strain B.megaterium BM 206, in a medium containing 163.1 μM/ml of acetochlor, after 12 days, the amount of pesticide decreased to 2.48 mkM/ml

The effect of phosphatemobilizers on the formation of the above-ground mass of corn plants
options	aboveground weight, g/plant
Control	10,25 ± 0,03
1. B. amyloliqufaciens IMB B – 7079	10,33 ± 0,05
2. B.polymyxa 1459 ЛЗ	12,50 ± 0,07
3. B.megaterium BМ 206	12,96 ± 0,02
4. B.megaterium BM ‑11 C	12,33 ± 0,05
5. B.megaterium 11 ПК ‑15-15	12,50 ± 0,03
6. Sample № 56	10,45 ± 0,06

The effect of various strains of phosphate-mobilizing agents on the accumulation of the aboveground mass of corn plants: К. Control (without processing) 1. Bacillus amyloliqufaciens IMB B - 7079; 2. B.polymyxa 1459 ЛС; 3. B.megaterium BМ 206; 4. B.megaterium BM 11 C; 5. B.megaterium 11 ПК - 15-15; 6. Sample № 56.

Effect of phosphatemobilizers on the formation of corn plant roots
options	mass of the root system, g/plant
Control (without inoculation)	9,50 ± 0,08
1. B. amyloliqufaciens IMB B – 7079	9,70 ± 0,06
2. B.polymyxa 1459 ЛЗ	18,36 ± 0,05
3. B.megaterium BМ 206	118,50 ± 0,11
4. B.megaterium BM ‑11 C	18,30 ± 0,05
5. B.megaterium 11 ПК ‑15-15	18,32 ± 0,04
6. Sample № 56	9,71 ± 0,07

The effect of various strains of phosphate mobilizers on the accumulation of the mass of the roots of corn plants: K. Control (without treatment) 1. Bacillus amyloliqufaciens IMB B - 7079; 2. B.polymyxa 1459 ЛС; 3. B.megaterium BМ 206; 4. B.megaterium BM 11 C; 5. B.megaterium 11 ПК - 15-15; 6. Sample № 56.

Effect of phosphatemobilizers on the content of chlorophylls a and b in the leaves of corn plants
options	chlorophyll, mg/per 100g
options	a	b	a+b
Control	88,3 ± 0,22	32,0 ± 0,55	120,3 ± 0,48
1. B. amyloliqufaciens IMB B – 7079	107,3 ± 0,19	39,0 ± 0,31	146,3 ± 0,35
2. B.polymyxa 1459 ЛЗ	137,2 ± 0,21	40,4 ± 0,29	177,6 ± 0,32
3. B.megaterium BМ 206	143,3 ± 0,39	43,1 ± 0,77	186,4 ± 0,57
4. B.megaterium BM ‑11 C	130,0 ± 0,24	35,8 ± 0,28	165,8 ± 0,36
5. B.megaterium 11 ПК ‑15-15	131,3 ± 0,29	36,1 ± 0,47	167,4 ± 0,34
6. Sample № 56	107,5 ± 0,58	28,5 ± 0,45	136,0 ± 0,42

The study of the activity of strains of phosphate mobilizers made it possible to determine the active phosphate mobilizer - B.megaterium ВМ 206, which is able to actively dissolve and, consequently, mobilize organic and inorganic forms of phosphorus.This testifies to its versatility.

The activity of phosphatemobile strains capable of dissolving inorganic phosphorus forms

(1. Bacillus amyloliqufaciens IMB B - 7079; 2. B.polymyxa 1459 ЛС; 3. B.megaterium BМ 206; 4. B.megaterium BM 11 C; 5. B.megaterium 11 ПК - 15-15).

Activity of strains of phosphate mobilizers capable of dissolving organic forms of phosphorus

(1. Bacillus amyloliqufaciens IMB B - 7079; 2. B.polymyxa 1459 ЛС; 3. B.megaterium BМ 206; 4. B.megaterium BM 11 C; 5. B.megaterium 11 ПК - 15-15).

practice

Combined use of the preparation Biophosphorin and the inoculant Biomag - Soy: — 2015 Demonstration Field "Soybean Century" (Kropyvnytskyi);
— Presowing treatment of soybean seeds of Bereginya variety;
— Complex of preparations TD LLC "Enzim - Agro": Biomag - soy (inoculant for soybean) - 2,8 л/т, BioMag - soy - 2,8 л/т, Biophosphorin - 1,5 л/т.

Influence of different processing options on the yield of soybean varieties Bereginya
option	productivity, t/ha	growth, t/ha
Control (without processing)	2,05	—
Biomag - Soy	2,14	+0,09
Biomag - SOY + Biophosphorin	2,22	+0,17

Processing seed corn: — 2017, LLC "Enselko - Agro" p.Sakhnovtsy (Khmelnytsky region);
— Pre-sowing processing of seed corn hybrid varieties Diadem;
— Biophosphorin - 1,5 л/т

Influence of pre-sowing seed treatment with Biophosphorin on corn yield
option	productivity, t/ha	growth, t/ha
Control (without processing)	8,6	—
Biophosphorin	9,34	+0,74

Spring barley seed treatment: — 2017, LLC Agrofirm Mayak, Kotelva (Poltava region);
— Presowing treatment of barley seeds of spring variety Svyatogor;
— Vial Trust - 0,4 l/t, Sistiva - 1,0 l/t, Biophosphorin - 1 l/t

Effect of pre-sowing seed treatment on yarley yarley yarch
option	yield, t/ha	growth, t/ha
Control (no processing)	3,2	—
Biofosphorin	4,0	+0,8

application

Method of processing and dosage of the preparation Biophosphorin
processing method	norm of application
Presowing seed treatment with suspension at the rate of 10 l/t	0,5–1,5 kg/ton of seeds
Spraying the soil to improve fertility (before cultivation, disking)	0,3 – 1,0 kg/ha
Fertigation	1-3 kg/ha (consumption of working solution up to 5000 l/ha)